Abstract
Recent investigations done in our laboratory on living cells by means of laser spectroscopy are reviewed. The first two are concerned with Chlorella: Stokes and anti-Stokes resonance Raman scatterings from carotenoid are measured to show that the Boltzmann distribution holds even in living cells. The fluorescence decay characteristics of chlorophyll are also studied by time-correlated single-photon counting under very weak light excitation. The remaining investigations are concerned with lymphocyte and are diagnostically important: The mechanism of fluorescence depolarization of fluorescein, which is known to show a change in the degree of polarization after antigenic stimulation, is clarified by time-resolved technique. Spectral properies of hematoporphyrin derivative in the cell, known as a sensitizer for recent photodynamic therapy, are also presented. Finally, the lineshape of organic dyes in solution is discussed with stochastic theories.
