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Volume 15, Issue 8
Displaying 1-9 of 9 articles from this issue
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Takashi KUSHIDA1987 Volume 15 Issue 8 Pages 584-585
Published: August 28, 1987
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (142K) -
Hiroyuki OHTANI, Takayoshi KOBAYASHI1987 Volume 15 Issue 8 Pages 586-594
Published: August 28, 1987
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSPicosecond and nanosecond spectroscopic studies on the primary processes of chromoproteins with retinal (retinoid proteins) are reviewed. The retinoid proteins described here are a visual pigment rhodopsin and two analogues, bacteriorhodopsin and sensory rhodpsin. Bacteriorhodopsin and sensory rhodopsin are a light-driven proton pump and a photoreceptor of the phototaxis of halobacteria. There are the following common charac-teristics in their photo-induced reactions. (i) Photoreactions are initiated by photoisomerizations of retinals and followed by sequential thermal reactions. (ii) In the early stage red-shifted intermediates are formed. They are converted to more blue-shifted intermediates in the sequential reactions.View full abstractDownload PDF (979K) -
Iwao YAMAZAKI1987 Volume 15 Issue 8 Pages 595-605
Published: August 28, 1987
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSTime-correlateds ingle-photonc ounting method combined with a synchronouslyp umped, cavity-dumped dye laser is now an essential technique for investigatingd ynamicalb ehavioro f biologicals ystems. Particularly, the use of a microchannel-platep hotomultiplier results in much higher time-resolutiont han conventional photomultipliers. Two examples of applicationt o studies of photosynthesisp rimaryp rocesses are shown co-ncerning (1) the sequentiale nergy transport in the phycobilina ntenna pigment system of some algae and (2) the reaction kinetics in reaction centers I and II in green alga, Chorella pyrenoidosa. A fluorescence band (F700) was, observed at 690-730 nm in the initial time region (0-180ps) along with the well-known spectrum (F685) of PSII-Chl a. The decay of F700 is governed by a fast energy transfer process from the antenna Chl a of PS I to P700 of RC I.View full abstractDownload PDF (1106K) -
Mikio YAMASHITA, Takahisa TOMONO, Shunsuke KOBAYASHI, Kenji TORIZUKA, ...1987 Volume 15 Issue 8 Pages 606-612
Published: August 28, 1987
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSBy using a highly sensitive streak-camera technique, we investigate incorporation and excretion processes of HpD into and from malignant tumor m-KSA cells in vitro. The picosecond time-dependent behavior of the fluorescence from HpD in the cells is measured as a function of the incubation and excretion times. The results show that the aggregate component of HpD which has a fast fluorescence lifetime of 100 ps and a red-shifted band of -660 nm in comparison with a monomer band selectively accumulates more andmore in the cells with the increase of the incubation time. Furthermore, it is shown that the aggregate excretes from the cells in the very different manner from the monomer.View full abstractDownload PDF (875K) -
Shuichi KINOSHITA, Takashi KUSHIDA1987 Volume 15 Issue 8 Pages 613-623
Published: August 28, 1987
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSRecent investigations done in our laboratory on living cells by means of laser spectroscopy are reviewed. The first two are concerned with Chlorella: Stokes and anti-Stokes resonance Raman scatterings from carotenoid are measured to show that the Boltzmann distribution holds even in living cells. The fluorescence decay characteristics of chlorophyll are also studied by time-correlated single-photon counting under very weak light excitation. The remaining investigations are concerned with lymphocyte and are diagnostically important: The mechanism of fluorescence depolarization of fluorescein, which is known to show a change in the degree of polarization after antigenic stimulation, is clarified by time-resolved technique. Spectral properies of hematoporphyrin derivative in the cell, known as a sensitizer for recent photodynamic therapy, are also presented. Finally, the lineshape of organic dyes in solution is discussed with stochastic theories.View full abstractDownload PDF (1153K) -
Takahiro KASUYA, Motowo TSUKAKOSHI1987 Volume 15 Issue 8 Pages 624-635
Published: August 28, 1987
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSThe exposure to a uv laser microbeam can modify a selected portion of cell membrane to be temporarily permiative, thereby allowing a foreign substance to enter living cell. As a first application of laser microbeam to the field of bionicis, the method was employed in incorporating the exogenous gene into cells. The method was found to offer a marked advantage over the existing ones in the success rate of gene incorporation, effi-ciency to large throughput, and in versatility to broad cell-type.View full abstractDownload PDF (4502K) -
Tatsuro SUZUKI, Yoshiaki HORIKAWA1987 Volume 15 Issue 8 Pages 636-646
Published: August 28, 1987
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSA real-times canning laser microscope for biological research is described, in which an acoust-optical deflector using TeO2 is used to observe the specimens in real time. The resolving power is 0.19μm when a He-Cd laser is used. High contrast clear images of nondyed bacilli or cells are obtained. Minute structures of a human chromosome which are hardly recognizable with a conventional optical microscope are clearly obserbed.View full abstractDownload PDF (3553K) -
Yoshio NOGUCHI, Yoshio TENJIN1987 Volume 15 Issue 8 Pages 647-656
Published: August 28, 1987
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSA cell sorter has become an important tool for chromosome purification and is used for constructing chromosome-specific gene libraries. New types of chromosome sorter have been developed, since conventional sorters cannot isolate whole types of human chromosomes and their sorting speed is relatively slow. This paperintroduces the flow cytometers recently developed, such as the Dual-Beam High-Speed sorter, the Triple-Lasersorter, the Fringe-Scan flow cytometer, and so on.View full abstractDownload PDF (1032K) -
Near-infrared CTMamoru TAMURA, Yasutomo NOMURA, Osamu HAZEKI1987 Volume 15 Issue 8 Pages 657-665
Published: August 28, 1987
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSThe principle of non-invasive optical measurement of tissue oxygenation was described with the special emphasis of near-infrared region. Near-infrared light is rather transparent to the living tissue. The profile of light scattering originated from the red blood cell suspension was measured. Using the picosecond spectroscopy, we measured the time of flight in the red blood cell suspension as well as living tissues. Based on the observations, we concluded that near-infrared imaging is feasible.View full abstractDownload PDF (786K)
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