抄録
As an approach to clarify biochemically the mechanism of intracellular killing of bacteria by phagocytic cells, the effect of several metabolic inhibitors on the bactericidal activity of mouse peritoneal macrophages (MP) on ingested Salmonella enteritidis and Escherichia coli was examined. 5×10-4M iodoacetate and 10-2M NaF added to the medium interfered significantly with the bactericidal activity of the MP, whereas sodium malonate, KCN, dinitrophenol, and anaerobiosis did not. Thus a close relation between the glycolytic pathway of MP and their bactericidal activity on ingested bacteria was demonstrated. However, oxamic acid and exclusion of external glucose, both of which significantly reduced lactate production by MP, did not affect the bactericidal activity of such cells. The lack of influence of puromycin on the bactericidal activity of MP excluded the possibility that de novo synthesis of lysosomal enzymes and bactericidal substances by the cells is necessary for accomplishment of the intracellular killing of bacteria. NaF inhibited significantly acid phosphatase and esterase activities, and iodoacetate slightly inhibited uricase activity, while sodium malonate, KCN and dinitrophenol did not exhibit any inhibitory effect on the activities of all the lysosomal enzymes examined. From these findings, the mechanism of the intracellular killing of the ingested bacteria by MP was discussed.
