Isolation of Polyribosome from Tobacco Plants Infected with Tobacco Mosaic Virus

Abstract

Polyribosomes have been isolated from tobacco leaves, Upon infection with TMV, a new polyribosome has appeared which is specific for infection, and TMV-antigenic protein is formed on this polyribosome. This new polyribosome has an S-value of 360-380. From these results, it is suggested that this TMV specific polyribosome is an aggregate of 60-80 ribosomes which is bound by TMV-RNA as messenger, and that TMV-RNA is translated polycistronically.