Intestinal Resistance in the Experimental Enteric Infection of Mice with a Mouse Adenovirus II. Determination of the Neutralizing Substance in the Intestinal Tract as an IgA Antibody

Kazuo HASHIMOTO; Masako YOSHIKAWA; Yumiko SUGIHARA; Shogo SASAKI

doi:10.1111/j.1348-0421.1971.tb00611.x

Intestinal Resistance in the Experimental Enteric Infection of Mice with a Mouse Adenovirus

II. Determination of the Neutralizing Substance in the Intestinal Tract as an IgA Antibody

Kazuo HASHIMOTO, Masako YOSHIKAWA, Yumiko SUGIHARA, Shogo SASAKI

著者情報

ジャーナルフリー

1971 年 15 巻 6 号 p. 499-508

DOI https://doi.org/10.1111/j.1348-0421.1971.tb00611.x

詳細

Published: 1971 Received: 1971/05/27 Available on J-STAGE: 2008/04/18 Accepted: - Advance online publication: - Revised: -
訂正情報
訂正日: 2008/04/18 訂正理由: - 訂正箇所: 著者情報訂正内容: Wrong : Sumio ARAI¹⁾, Yorio HINUMA¹⁾²⁾, Keizo MATSUMOTO¹⁾, Takashi NAKAMURA¹⁾
Right : Kazuo HASHIMOTO¹⁾, Masako YOSHIKAWA¹⁾, Yumiko SUGIHARA¹⁾, Shogo SASAKI¹⁾

訂正日: 2008/04/18 訂正理由: - 訂正箇所: 所属機関情報訂正内容: 訂正前 :
1) Department of the First Internal Medicine, School of Medicine, and Department of Microbiology, School of Dentistry, Tohoku University
2) Department of Microbiology, Kumamoto University Medical School

訂正後 :
1) Department of Microbiology, Keio University School of Medicine

訂正日: 2008/04/18 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/04/18 訂正理由: - 訂正箇所: 論文タイトル訂正内容: Wrong : Delayed Type Hypersensitivity in Hamsters Infected with Mycoplasma pneumoniae as Revealed by Macrophage Migration Inhibition Test
Right : Intestinal Resistance in the Experimental Enteric Infection of Mice with a Mouse Adenovirus

訂正日: 2008/04/18 訂正理由: - 訂正箇所: 論文サブタイトル訂正内容: Right : II. Determination of the Neutralizing Substance in the Intestinal Tract as an IgA Antibody

訂正日: 2008/04/18 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : A delayed type hypersensitivity was demonstrated in hamsters infected with a fresh isolate of Mycoplasma pneumoniae by the following two procedures of macrophage migration inhibition test. In the first procedure, inhibition of migration of peritoneal exudate cells from normal guinea pigs by the addition of splenic cells from infected hamsters in the presence of the mycoplasma antigen was examined. In the second procedure, migration of peritoneal exudate cells, composed of macrophages and lymphocytes, from infected hamsters was examined after the addition of the antigen. Virtually, both procedures gave a similar result, suggesting the presence of cell-mediated immunity in the infected animals. In these infected hamsters, humoral antibody responses were also demonstrated at the same time.
Right : In order to find out whether the mouse adenovirus-neutralizing substance, which appeared in the intestinal tract of mice orally infected with mouse adenovirus, was an immunoglobulin, examinations were carried out for the status of 3 classes of immunoglobulin, IgA, IgG, and IgM, in the intestinal tract as well as in the serum of the mouse. In infected mice, as in uninfected mice, the serum contained much IgG, a moderate amount of IgA, and a small amount of IgM, whereas the intestinal wall showed a moderate amount of IgA, a small amount of IgG and no IgM, and the intestinal contents contained a moderate amount of IgA. Secondly, DEAE-cellulose chromatography or Sephadex G-200 gel filtration was done in order to know whether the virus-neutralizing activity was recoverable in the fractions containing some class of immunoglobulin. The result indicated that a large part of the activity in the serum was recovered in the fractions of IgG and a small part in those of IgA. In the case of the intestinal wall, a large part of the activity was found in the fractions of IgA, and only a small part in the fractions containing both IgG and IgA. In the intestinal contents, the activity was detected solely in the fractions containing IgA. Finally, when the substance from the intestinal wall was purified by DEAE and Sephadex, a parallel increase of both IgA and the virus-neutralizing activity per protein content was observed. Thus, it became clear that the mouse adenovirus-neutralizing substance in the intestinal tract was an antibody against the virus, and that it mostly belongs to IgA.

訂正日: 2008/04/18 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : [1] Chanock, R. M., James, W. D., Fox, H. H., Turner, H. C., Mufson, H. A., and Hay-flik, L. 1962. Growth of Eaton PPLO in broth and preparation of complement fix-ing antigen. Proc. Soc. Exp. Biol. Med. 110: 884-889.
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訂正日: 2008/04/18 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

In order to find out whether the mouse adenovirus-neutralizing substance, which appeared in the intestinal tract of mice orally infected with mouse adenovirus, was an immunoglobulin, examinations were carried out for the status of 3 classes of immunoglobulin, IgA, IgG, and IgM, in the intestinal tract as well as in the serum of the mouse. In infected mice, as in uninfected mice, the serum contained much IgG, a moderate amount of IgA, and a small amount of IgM, whereas the intestinal wall showed a moderate amount of IgA, a small amount of IgG and no IgM, and the intestinal contents contained a moderate amount of IgA. Secondly, DEAE-cellulose chromatography or Sephadex G-200 gel filtration was done in order to know whether the virus-neutralizing activity was recoverable in the fractions containing some class of immunoglobulin. The result indicated that a large part of the activity in the serum was recovered in the fractions of IgG and a small part in those of IgA. In the case of the intestinal wall, a large part of the activity was found in the fractions of IgA, and only a small part in the fractions containing both IgG and IgA. In the intestinal contents, the activity was detected solely in the fractions containing IgA. Finally, when the substance from the intestinal wall was purified by DEAE and Sephadex, a parallel increase of both IgA and the virus-neutralizing activity per protein content was observed. Thus, it became clear that the mouse adenovirus-neutralizing substance in the intestinal tract was an antibody against the virus, and that it mostly belongs to IgA.

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