Electron Microscopic Studies on the Replication of Ibaraki Virus

抄録

Ibaraki virus is an agent of epizootic cattle disease resembling bluetongue. The virus was purified from infected BHK-21 cells and bovine kidney cells. The particle negatively stained was icosahedral and had a diameter of 45-50mμ. By electron microscopy, the virus was found in the cytoplasm of cells, assembling in lysosomal or matrix-like dense bodies. In early stages of infection, 2 to 7hr, virus particles were located in vesicles, simulating a viropexis, whereas at 24 to 30hr, after infection, a large number of cores of progeny virus were scattered in matrix-like bodies or packed and crystallized in lysosomal bodies bounded by a membrane. Sometimes peculiar tubular structures were found near the matrix-like bodies. The relation-ship between these structures and vinus replication was not clear. The morphological features of the particles and virus development in cells resembled those of viruses of bluetongue, African horse-sickness. Colorado tick fever and reo.