Japanese Journal of Microbiology
Print ISSN : 0021-5139
Studies on Temperate Phages of Lactobacillus salivarius
I. Morphological, Biological, and Serological Properties of Newly Isolated Temperate Phages of Lactobacillus salivarius
Kiyoshi TOHYAMAToshizo SAKURAIHiroshi ARAIAkira ODA
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1972 年 16 巻 5 号 p. 385-395

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Twenty-three temperate phages of Lactobacillus salivarius isolated from human feces were studied as to their morphological, biological, and serological properties. (1) Among 30 strains of L. salivarius tested, 23 strains were lvscd by induction with mitomycin C (MC). In all these lysates, phage particles were detected by electron mioroscopic cxamination. (2) These phages were morphologically divided into three groups: particles with a regular hexagonal tead and a long flexible tail; particles having a regular hexagonal head with or without a short tail-like structure: particles with an elongated head and a long noncontractile tail. (3) Only two, phage 223 having an elongated head and phage 227 with a regular hexagonal head and a long noncontractile tail, produced tiny and very turbid plaques on several host bacteria_Six phages could produce only inhibition zones, rangnig from complete inhibition through partial inhibition to normal growth by a serial dilution spot test. (4) All these killer paiticles could also inhibit the growth of their producer cells. (5) .A serologieal relationship was observed between temperate phages and killer particles, and this was somewhat consistent with the morphological groupings.
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