SOME PHYSIOLOGICAL ASPECTS OF MICROCOCCUS

II. EXPERIMENTS WITH CELL-FREE EXTRACT

Abstract

1. The cell-free extract of Micrococcus citreus oxidized the citric acid cycle intermediates and related compounds except acetate, aspartate, pyruvate and glucose.
2. The cell-free extract of M. pyogenes var. aureus (Terashima) oxidized these compounds except citrate, acetate, asparate, pyruvate and glucose.
3. The cell-free extract of M. pyogenes var. albus showed no noticeable oxygen consumption in the presence of these compounds except the very low ones in a-ketoglutarate, succinate, malate and alanine.
4. By differential centrifugation, the glutamate-oxidizing enzyme system was divided into two fractions, the soluble and particulate fractions.
5. The particulate fraction oxidized malate by itself.
6. Glutamic-pyruvic and aspartic-a-ketoglutaric transaminations were car-ried out by the soluble fraction of M. pyogenes var. aureus (Terashima)