PROTOPLAST FORMATION AND MANDELIC ACID DEHYDROGENASE LOCALIZATION IN PSEUDOMONAS TLUORESCENS

Abstract

Protoplasts of Pseudomonas fluorescens A. 3.12. were obtained by the use of lysozyme and ethylenediaminetetraacetic acid in a alkaline hypertonic medium.
Mandelic acid dehydrogenase, which is known to be a particulate-bound enzyme, was recovered in the ghost fraction.