STUDIES ON A NEW METHOD FOR THE INCOMPLETE ANTIBODY IN TUBERCULOSIS

TIUZI SINDO; TERUKAZU UTAHASHI; KUNIO HAGA; AYAO HIYAMA; MICHIO ITOI; YOICHI ICHIKAWA

doi:10.1111/j.1348-0421.1958.tb00067.x

TIUZI SINDO, TERUKAZU UTAHASHI, KUNIO HAGA, AYAO HIYAMA, MICHIO ITOI, YOICHI ICHIKAWA

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1958 Volume 2 Issue 2 Pages 159-166

DOI https://doi.org/10.1111/j.1348-0421.1958.tb00067.x

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Published: 1958 Received: February 21, 1958 Available on J-STAGE: April 18, 2008 Accepted: - Advance online publication: - Revised: -
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Date of correction: April 18, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : 1) Twenty samples of maternal sera were examined by the hemagglutination and hemolytic tests for antituberculous antibody. Sixteen out of 20 samples were positive and 4 negative in the former; 12 were positive and 8 negative in the latter.
Twenty samples of umbilical cord sera were also tested for both reactions. The hemagglutination test was negative in all samples while 12 out of 20 samples were positive in the hemolytic test.
It is considered that the specific complete tuberculous antibody which is thought to play a role in Middlebrook-Dubos' hemagglutination can not pass through placenta. At the same time, it is suggested by the present study that there may exist a correlation between the antibody tested in the hemolytic reaction and the incomplete antibody. However, it cannot be said at the present time that the specific complete antibody is unable to participitate in the hemolytic reaction.
2) A method for the detection of the incomplete antibody in tuberculosis has been devised by combining the Middlebrook-Dubos' test using sheep red cells sensitized with tuberculin fraction and the indirect Coombs' test.
Twelve positive and 8 negative samples of umbilical cord sera in the hemolytic test were examined by the new method. Out of 12 positive samples, 5 showed clear cut agglutination, the degree of the reaction being nearly parallel to that of hemolytic reaction; 5 showed slight agglutination; and 2 were negative. Although the incomplete tuberculous antibody is considered to be present in the 2 negative samples, it may not be measured due to the limits of the technique used. Eight negative samples in the hemolytic test were negative also in this method.
Therefore, tuberculous antibody appears to pass through the placenta in the form of incomplete antibody. As Coombs' test is one of the specific means among several techniques for the detection of incomplete antibody, the new method (Sindo and Utahashi's method)* is considered to afford a reliable means of verifying the specific incomplete antibody content of sera by the use of sensitized red cells.
Right : 1) Twenty samples of maternal sera were examined by the hemagglutination and hemolytic tests for antituberculous antibody. Sixteen out of 20 samples were positive and 4 negative in the former; 12 were positive and 8 negative in the latter.
Twenty samples of umbilical cord sera were also tested for both reactions. The hemagglutination test was negative in all samples while 12 out of 20 samples were positive in the hemolytic test.
It is considered that the specific complete tuberculous antibody which is thought to play a role in Middlebrook-Dubos' hemagglutination can not pass through placenta. At the same time, it is suggested by the present study that there may exist a correlation between the antibody tested in the hemolytic reaction and the incomplete antibody. However, it cannot be said at the present time that the specific complete antibody is unable to participitate in the hemolytic reaction.
2) A method for the detection of the incomplete antibody in tuberculosis has been devised by combining the Middlebrook-Dubos' test using sheep red cells sensitized with tuberculin fraction and the indirect Coombs' test.
Twelve positive and 8 negative samples of umbilical cord sera in the hemolytic test were examined by the new method. Out of 12 positive samples, 5 showed clear cut agglutination, the degree of the reaction being nearly parallel to that of hemolytic reaction; 5 showed slight agglutination; and 2 were negative. Although the incomplete tuberculous antibody is considered to be present in the 2 negative samples, it may not be measured due to the limits of the technique used. Eight negative samples in the hemolytic test were negative also in this method.
Therefore, tuberculous antibody appears to pass through the placenta in the form of incomplete antibody. As Coombs' test is one of the specific means among several techniques for the detection of incomplete antibody, the new method (Sindo and Utahashi's method) is considered to afford a reliable means of verifying the specific incomplete antibody content of sera by the use of sensitized red cells.

Date of correction: April 18, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

1) Twenty samples of maternal sera were examined by the hemagglutination and hemolytic tests for antituberculous antibody. Sixteen out of 20 samples were positive and 4 negative in the former; 12 were positive and 8 negative in the latter.
Twenty samples of umbilical cord sera were also tested for both reactions. The hemagglutination test was negative in all samples while 12 out of 20 samples were positive in the hemolytic test.
It is considered that the specific complete tuberculous antibody which is thought to play a role in Middlebrook-Dubos' hemagglutination can not pass through placenta. At the same time, it is suggested by the present study that there may exist a correlation between the antibody tested in the hemolytic reaction and the incomplete antibody. However, it cannot be said at the present time that the specific complete antibody is unable to participitate in the hemolytic reaction.
2) A method for the detection of the incomplete antibody in tuberculosis has been devised by combining the Middlebrook-Dubos' test using sheep red cells sensitized with tuberculin fraction and the indirect Coombs' test.
Twelve positive and 8 negative samples of umbilical cord sera in the hemolytic test were examined by the new method. Out of 12 positive samples, 5 showed clear cut agglutination, the degree of the reaction being nearly parallel to that of hemolytic reaction; 5 showed slight agglutination; and 2 were negative. Although the incomplete tuberculous antibody is considered to be present in the 2 negative samples, it may not be measured due to the limits of the technique used. Eight negative samples in the hemolytic test were negative also in this method.
Therefore, tuberculous antibody appears to pass through the placenta in the form of incomplete antibody. As Coombs' test is one of the specific means among several techniques for the detection of incomplete antibody, the new method (Sindo and Utahashi's method) is considered to afford a reliable means of verifying the specific incomplete antibody content of sera by the use of sensitized red cells.

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