ONCOLYTIC EFFECT OF NEWCASTLE DISEASE VIRUS ON YOSHIDA SARCOMA (I)

Abstract

The effect of NDV on Yoshida sarcoma in vivo and in vitro was examined, and the following results were obtained:
1. The oncolytic effect of NDV against Yoshida sarcoma by implantation of mixture of tumor cells and NDV was confirmed.
2. When 10^-4 diluted virus was used, oncolysis did not occur, but when NDV original, 10^-1, 10^-2 diluted and concentrated was used, the Yoshida sarcoma oncolysis was observed.
3. Oncolysis was confirmed when virus inoculation was performed on the 2nd day after tumor implantation.
4. Even when NDV was inoculated continuously for a week, there was no further oncolysis from a single inoculation.
5. In case of subcutaneous implantations of tumor cells and NDV mixture, there were no remarkable differences upon tumor growth between virus added groups and tumor control groups.
6. The Yoshida sarcoma oncolysis did not occur with inactivated NVD.
7. The Yoshida sarcoma oncolysis was also shown by NDV in mice.
8. No propagation of NDV on Yoshida sarcoma was shown in vivo.
9. The Yoshida sarcoma cell destruction by NDV also occurred in tissue culture. The amount of cell destruction depended on the virus concentrations. In vitro, virus multiplication was demonstrated 72 hours after inoculation.