AMINO ACID INCORPORATION IN THE SUBCELLULAR STRUCTURES OF PSEUDOMONAS FLUORESCENS A3-12

I. THE INCORPORATION SITE OF S35-METHIONINE IN THE INTACT CELLS

Abstract

The distributions of S³⁵-methionine incorporated into the subcellular fractions of Pseudomonas fluorescens A3-12, which was forced to synthesize some inducible enzymes, were studied. The cells were prompted to form l-mandelic acid oxidase, benzoic acid oxidase, or pyrocatecase.
It could concluded that the insoluble fraction prepared by the ‘direct lysis’ of bacteria plays an important role in the incorporation of amino acids.
The opposite relationship in distribution of S³⁵-methionine between the insoluble and the soluble fraction for mandelic acid oxidase and pyrocatecase formations was discussed.