STUDIES ON THE PURINE REQUIREMENTS OF VIBRIO EL TOR

Abstract

Using semisynthetic media, experiments were carried out on the nutritional requirements of Vibrio El Tor, and the following results were obtained.
1. Ammonium salts could not be utilized as the nitrogen source for both strains.
2. A 0.1% glucose concentration was adequate.
3. A M/40 phosphate concentration was adequate and the addition of less than 100 γ/ml MgSO₄•7H₂O enhanced its growth.
4. The addition of 7 vitamins into a semisynthetic medium inhibited its growth.
5. Each of 3 purines, adenine, guanine and xanthine, remarkably enhanced its early growth, and there were scarcely any differences among them. Also, adenosine had the same degree of stimulatory effect.
6. The production of hemolysin in the culture filtrate was influenced by theaddition of the purine, and in broth culture. The hemolytic titer was higher despite an inferior growth of the organism.