Abstract
The cytotoxic activity of guinea pig lymphotoxin (GLT) was increased. when the target cells were treated with the following metabolic inhibitors : puromycin, actinomycin D, NaN3, NaF, and 2, 4-dinitrophenol, whereas calf serum, N-acetyl-D-galactosamine, colchicine, and vinblastin inhibited GLT-cytotoxicity. Puromycin, an inhibitor of protein synthesis, exhibited the most potent enhancing effect on GLT-cytotoxicity. It seems likely that there are certain cellular metabolic processes depending on cellular protein synthesis which antagonize GLT-cytotoxicity. Taking advantage of this effect of puromycin, a time-saving assay method for GLT-cytotoxicity was developed.
