Growth of Poliovirus in HeLa Cells Persistently Infected with HVJ (Sendai Virus)

抄録

The growth of poliovirus in a HeLa cell culture persistently infected with the hemagglutinating virus of Japan (HVJ, the Sendai strain of parainfluenza 1 virus) (HeLa_HVJ) was studied. Plaques produced by poliovirus on HeLa_HVJ cell monolayers were hazier, smaller and fewer than those on HeLa cells. HeLa_HVJcells were indistinguishable from normal HeLa cells with respect to adsorption rate and penetration efficiency of poliovirus. Extracellular yields of poliovirus in HeLa_HVJ cells were lower, and the cytopathic changes were less than those in normal HeLa cells, while cell-associated virus growth in HeLa_HVJ cells was nearly equal to that in HeLa cells. HeLa_HVJ cells responded more effectively to the action of magnesium chloride, which facilitates virus release from infected cells, resulting in an increase of virus yield to the level of normal HeLa cells, with an early appearance of cytopathic effects. No reduction in poliovirus yield could be detected in HeLa cells acutely infected with HVJ. The relationship between the inhibition of the release of poliovirus from HeLa_HVJ cells and the persistent infection of the cells with HVJ is discussed.