抄録
We established a new method for detecting enteropathogenic Escherichia coli adhering to HEp-2 cells. An essential part of the method is an assay of β-galactosidase activity of adhered bacterial cells. It consisted of the following steps: (1) culture of bacterial cells in a medium containing isopropyl-thio-β-D-galactoside, an inducer of β-galactosidase; (2) incubation of a bacterial culture with monolayered HEp-2 cells in a 96-well culture plate; (3) washing wells to remove bacterial cells which did not adhere to HEp-2 cells, and (4) enzymic reaction for β-galactosidase assay in wells. The strains used differed widely in their β-galactosidase activities. However, a calibration curve for the enzyme activity, obtained from each bacterial sample, showed that 105 bacteria per well permitted an accurate estimation. The enzyme activity of adhered bacteria to the monolayered cells showed that 107 bacteria were appropriate for the adherence assay. The number of adhered bacteria thus obtained was in good agreement with a viable cell count. The result indicates that the new method is more reliable than a widely used method, counting the number of bacteria under a microscope. The present method also makes it easy to detect adherent strains of E. coli in large numbers of specimens.
