Nosocomial Diarrhoea in the Elderly Due to Enterotoxigenic Clostridium perfringens

Akihito Wada; Yoshishige Masuda; Makiko Fukayama; Tsutomu Hatakeyama; Yoshitoki Yanagawa; Haruo Watanabe; Takashi Inamatsu

Nosocomial Diarrhoea in the Elderly Due to Enterotoxigenic Clostridium perfringens

Akihito Wada, Yoshishige Masuda, Makiko Fukayama, Tsutomu Hatakeyama, Yoshitoki Yanagawa, Haruo Watanabe, Takashi Inamatsu

著者情報

キーワード: Nosocomial diarrhoea, Enterotoxigenic Clostridium perfringens, Reverse passive latex agglutination assay, Pulsed-field gel electrophoresis

ジャーナルフリー

1996 年 40 巻 10 号 p. 767-771

詳細

Published: 1996/10/20 Received: 1996/01/12 Available on J-STAGE: 2008/03/17 Accepted: 1996/07/16 Advance online publication: - Revised: -
訂正情報
訂正日: 2008/03/17 訂正理由: - 訂正箇所: 論文タイトル訂正内容: Wrong : Nosocomial Diarrhoea in the Elderly Due to Enterotoxigenic Clostridium perfringens
Right : Nosocomial Diarrhoea in the Elderly Due to Enterotoxigenic Clostridium perfringens

訂正日: 2008/03/17 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : To diagnose sporadic diarrhoea due to Clostridium perfringens infection, faecal specimens from elderly patients were examined directly for C. perfringens enterotoxin using reverse passive latex agglutination assay, and then cultured for this organism. C. perfringens isolates from those samples were grouped by slide agglutination and by pulsed-field gel electrophoresis (PFGE). Fifty of the 60 isolates agglutinated with newly raised antiserum WX2 and 38 shared the same genomic PFGE pattern. Characteristics of the epidemics and experimental data suggest that the diarrhoea was caused by a nosocomial spread of C. perfringens, and not by a food-borne outbreak.
Right : To diagnose sporadic diarrhoea due to Clostridium perfringens infection, faecal specimens from elderly patients were examined directly for C. perfringens enterotoxin using reverse passive latex agglutination assay, and then cultured for this organism. C. perfringens isolates from those samples were grouped by slide agglutination and by pulsed-field gel electrophoresis (PFGE). Fifty of the 60 isolates agglutinated with newly raised antiserum WX2 and 38 shared the same genomic PFGE pattern. Characteristics of the epidemics and experimental data suggest that the diarrhoea was caused by a nosocomial spread of C. perfringens, and not by a food-borne outbreak.

訂正日: 2008/03/17 訂正理由: - 訂正箇所: 発行日訂正内容: 訂正後 : 19961020

訂正日: 2008/03/17 訂正理由: - 訂正箇所: キーワード情報訂正内容: Wrong : Nosocomial diarrhoea, Enterotoxigenic Clostridium perfringens, Reverse passive latex agglutination assay, Pulsed-field gel electrophoresis
Right : Nosocomial diarrhoea, Enterotoxigenic Clostridium perfringens, Reverse passive latex agglutination assay, Pulsed-field gel electrophoresis

訂正日: 2008/03/17 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1) Allen, S.D. 1985. Clostridium, p. 434-444. In Lennette, E.H., Balows, A., Hausler, W.J., Jr., and Shadomy, H.J. (eds), Manual of clinical microbiology, 4th ed, American Society for Microbiology, Washington, D.C.
2) Bartlett, J.G. 1988. Gas gangrene (other clostridium-associated diseases), p. 1850-1860. In Mandell, G., Douglas, R.G., and Bennett, J.E. (eds), Infectious diseases, 3rd ed, Churchill Livingstone, New York.
3) Borriello, S.P., Larson, H.E., Welch, A.R., and Barclay, F. 1984. Enterotoxigenic Clostridium perfringens: a possible cause of antibiotic-associated diarrhoea. Lancet i: 305-307.
4) Borriello, S.P., Barclay, F.E., Welch, A.R., Stringer, M.F., Watson, G.N., Williams, R.K.T., Seal, D.V., and Sullen K. 1985. Epidemiology of diarrhoea caused by enterotoxigenic Clostridium perfringens. J. Med. Microbiol. 20: 363-372.
5) Canard, B., and Cole, S.T. 1989. Genome organization of the anaerobic pathogen Clostridium perfringens. Proc. Natl. Acad. Sci. U.S.A. 86: 6676-6680.
6) Canard, B., Saint-Joanis, B., and Cole, T. 1992. Genomic diversity and organization of virulence genes in the pathogenic anaerobe Clostridium perfringens. Mol. Microbiol. 6:1421-1429.
7) Clabots, C.R., Bettin, K.M., Peterson, L.R., and Gerding, D.N. 1991. Evaluation of cycloserine-cefoxitin-fructose agar and cycloserine-cefoxitin-fructose broth for recovery of Clostridium difficile from environmental sites. J. Clin. Microbiol. 29: 2633-2635.
8) Itoh, T. 1972. Incidence of heat resistant Clostridium perfringens in feces of healthy subjects, serotyping of isolates, and food poisoning caused by new serotype organisms. Annual Report of Tokyo Metropolitan Research Laboratory of Public Health 34: 7-39.
9) Jackson, S.G., Yip-Chuck, D.A., Clark, J.B., and Brodsky, M.H. 1986. Diagnostic importance of Clostridium perfringens enterotoxin analysis in recurring enteritis among elderly, chronic care psychiatric patients. J. Clin. Microbiol. 23: 748-751.
10) Larson, H.E., and Borriello, S.P. 1988. Infectious diarrhea due to Clostridium perfringens. J. Infect. Dis. 157: 390-391.
11) Ohtani, Y., and Ujiiye, A. 1987. Sporulation and enterotoxin production of Clostridium perfringens in a modified DS medium. J. Food Hyg. Soc. Jpn. 28: 281-285.
12) Stringer, M.F., Turnbull, P.C.B., and Gilbert, R.J. 1980. Application of serological typing to the investigation of outbreaks of Clostridium perfringens food poisoning, 1970-1978. J. Hyg. 8: 443-456.
13) Williams, R., Piper, M., Borriello, P., Barclay, F., Welch, A., Seal, D., and Sullens, K. 1985. Diarrhoea due to enterotoxigenic Clostridium perfringens: clinical features and management of a cluster of ten cases. Age Ageing 14: 296-302.
14) Yamagishi, T., Serikawa, T., Morita, R., Nakamura, S., and Nishida, S. 1976. Persistent high numbers of Clostridium perfringens in the intestine of Japanese aged adults. Jpn. J. Microbiol. 20: 397-403.

Right : 1) Allen, S.D. 1985. Clostridium, p. 434-444. In Lennette, E.H., Balows, A., Hausler, W.J., Jr., and Shadomy, H.J. (eds), Manual of clinical microbiology, 4th ed, American Society for Microbiology, Washington, D.C.
2) Bartlett, J.G. 1988. Gas gangrene (other clostridium-associated diseases), p. 1850-1860. In Mandell, G., Douglas, R.G., and Bennett, J.E. (eds), Infectious diseases, 3rd ed, Churchill Livingstone, New York.
3) Borriello, S.P., Larson, H.E., Welch, A.R., and Barclay, F. 1984. Enterotoxigenic Clostridium perfringens : a possible cause of antibiotic-associated diarrhoea. Lancet i : 305-307.
4) Borriello, S.P., Barclay, F.E., Welch, A.R., Stringer, M.F., Watson, G.N., Williams, R.K.T., Seal, D.V., and Sullens, K. 1985. Epidemiology of diarrhoea caused by enterotoxigenic Clostridium perfringens. J. Med. Microbiol. 20 : 363-372.
5) Canard, B., and Cole, S.T. 1989. Genome organization of the anaerobic pathogen Clostridium perfringens. Proc. Natl. Acad. Sci. U.S.A. 86 : 6676-6680.
6) Canard, B., Saint-Joanis, B., and Cole, T. 1992. Genomic diversity and organization of virulence genes in the pathogenic anaerobe Clostridium perfringens. Mol. Microbiol. 6 : 1421-1429.
7) Clabots, C.R., Bettin, K.M., Peterson, L.R., and Gerding, D.N. 1991. Evaluation of cycloserine-cefoxitin-fructose agar and cycloserine-cefoxitin-fructose broth for recovery of Clostridium difficile from environmental sites. J. Clin. Microbiol. 29 : 2633-2635.
8) Itoh, T. 1972. Incidence of heat resistant Clostridium perfringens in feces of healthy subjects, serotyping of isolates, and food poisoning caused by new serotype organisms. Annual Report of Tokyo Metropolitan Research Laboratory of Public Health 34 : 7-39.
9) Jackson, S.G., Yip-Chuck, D.A., Clark, J.B., and Brodsky, M.H. 1986. Diagnostic importance of Clostridium perfringens enterotoxin analysis in recurring enteritis among elderly, chronic care psychiatric patients. J. Clin. Microbiol. 23 : 748-751.
10) Larson, H.E., and Borriello, S.P. 1988. Infectious diarrhea due to Clostridium perfringens. J. Infect. Dis. 157 : 390-391.
11) Ohtani, Y., and Ujiiye, A. 1987. Sporulation and enterotoxin production of Clostridium perfringens in a modified DS medium. J. Food Hyg. Soc. Jpn. 28 : 281-285.
12) Stringer, M.F., Turnbull, P.C.B., and Gilbert, R.J. 1980. Application of serological typing to the investigation of outbreaks of Clostridium perfringens food poisoning, 1970-1978. J. Hyg. 84 : 443-456.
13) Williams, R., Piper, M., Borriello, P., Barclay, F., Welch, A., Seal, D., and Sullens, K. 1985. Diarrhoea due to enterotoxigenic Clostridium perfringens : clinical features and management of a cluster of ten cases. Age Ageing 14 : 296-302.
14) Yamagishi, T., Serikawa, T., Morita, R., Nakamura, S., and Nishida, S. 1976. Persistent high numbers of Clostridium perfringens in the intestine of Japanese aged adults. Jpn. J. Microbiol. 20 : 397-403.

抄録

To diagnose sporadic diarrhoea due to Clostridium perfringens infection, faecal specimens from elderly patients were examined directly for C. perfringens enterotoxin using reverse passive latex agglutination assay, and then cultured for this organism. C. perfringens isolates from those samples were grouped by slide agglutination and by pulsed-field gel electrophoresis (PFGE). Fifty of the 60 isolates agglutinated with newly raised antiserum WX2 and 38 shared the same genomic PFGE pattern. Characteristics of the epidemics and experimental data suggest that the diarrhoea was caused by a nosocomial spread of C. perfringens, and not by a food-borne outbreak.

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