A New Method for the Sequence Determination of a Peptide Mixture through Molecular Weight Measurements by Mass Spectrometry

Abstract

The amino acid sequence of a peptide is determined by the combination of the Edman degradation and the field desorption mass spectrometry. The kind of the PTH amino acid is determined by measuring the molecular weights of the residual intact peptides before and after the degradation. The molecular weights are determined from the (M+H)⁺ peaks of FD mass spectra. The sequence determinations of the peptide fragments in a peptide mixture can be done parallelly and simultaneously by this method.