抄録
Among various methods, hyphenated mass spectrometry combined with gas chromatography and liquid chromatography has been well recognized as a powerful tool for the separatory determination of biologically active substances. The review presents the clean-up methods for sensitive determination of drugs and their metabolites in complex matrices such as blood and urine. The state of the art of solid-phase extraction using traditional sorbents and immunoaffinity extraction by an immobilized antibody column as well as chromatographic separation by lipophilic ion-exchange gel are discussed.
