Use of Nano Liquid Chromatography/Nanoelectrospray Ionization/Ion Trap Mass Spectrometer for Proteome Quantitation and Identification

Abstract

We describe the potential and applicability of ion trap mass spectrometer to high-throughput expression proteome analysis. In an ion trap, the masses of the peptide ions are analyzed, and/or a particular ion can be selected to undergo collisions resulting in its fragmentation by which sequence information can be directly obtained. Equipped with nanoflow capillary liquid chromatography directly interfaced with nanoelectrospray ionization, ion trap mass spectrometer has been one of the standard systems to identify and quantify large numbers of proteins in complex protein mixtures. The characteristics including the stability, reliability and capability of MSⁿ essentially lead the mass spectrometer to the center of proteome-wide analysis.