抄録
In general, it is difficult to distinguish between sulfate and phosphate groups in natural products, because these groups are found only in trace amounts in natural products. We have devised a hydrogen/deuterium (H/D) exchange method using the liquid chromatography mass spectrometry technique for establishing a general methodology to distinguish between sulfated and phosphorylated compounds. Sulfate- or phosphate-containing compounds were differentiated by the H/D exchange method based on the number of exchangeable hydrogen in deuterated sulfate (-SO3D) and phosphate (-PO3D2), respectively. In this paper, we describe an application of this method to a natural product and in particular to Watasenia oxyluciferin that is found in the firefly squids (Hotaru-ika in Japanese) for the purpose of identifying coelenteramide disulfate.
