To prevent the infectious disease from spreading, hand hygiene such as hand washing or using disinfections is one of the most effective ways to cut off the route of transmission. In this study, we evaluated the virucidal activity of benzalkonium chloride solution with and without certain additives. Our findings suggested that the inclusion of a specific amount of lauryl amine oxide did not significantly affect the effectiveness of benzalkonium chloride, unlike polyoxyethylene lauryl ethers and hydrogenated castor oil. It was also demonstrated that commercially available sanitizers containing 0.05 w/v% benzalkonium chloride showed more than 4 log10 reduction against influenza A virus (H1N1) and SARS-CoV-2 even without ethanol. As a result, using appropriate disinfection is effective measure to reduce the risk of infection.
