Liposome-Assisted Refolding of Microbial Transglutaminase

Abstract

The liposome-assisted refolding of recombinant microbial transglutaminase (MTG) was achieved by using the dilution method. The analysis of the partly-denatured MTG by using the calcein-trapping immobilized liposome chromatography showed the interaction between the MTG and liposome under the above condition. The effect of the addition of liposome was then investigated, resulting that the refolding yield of MTG was improved from 66% at control to 82% by adding the liposome with suitable membrane fluidity and surface charge density.