Abstract
Recently, we developed novel liposomal nanobubbles (Bubble liposomes) containing perfluoropropane, which is an ultrasound imaging gas. Here, we used ultrasound to induce cavitation in Bubble liposomes and then investigated their ability to deliver gene and drug. The combination of Bubble liposomes and ultrasound could deliver plasmid DNA into many cell types without cytotoxicity. Additionally, in cancer gene therapy, this system could effectively deliver interleukin–12 (IL–12) corded plasmid DNA into tumor tissue and effective anti–tumor effect was achieved. Moreover, this system could be utilized as an effective anti–cancer agent delivery system into the cancer cells which had the resistance for the agent. Thus, this system might be a novel and efficient gene and drug delivery system.
