抄録
Tissue regeneration using biodegradable scaffolds such as poly(L–lactic acid) (PLLA) has been attracting great attention as a new alternating strategy to the artificial organs and organ transplantation. However, PLA is not necessarily an ideal substance for the scaffold from the viewpoint of the cell–compatibility and selectivity. Moreover, it is quite difficult to modify the PLA surfaces because of the lack of side functional groups. In the present study, we developed a novel immobilization method of bioactive molecules onto PLLA nanofiber membrane. Amphiphilic block oligomers probes composed of oligo(lactic acid) (OLA) and bioactive peptides were newly synthesized (OLA–peptide), and a small amount of the synthesized OLA–peptides was added to PLA solution before fabricating the PLA nanofiber membrane in order to impart the specific cell adhesion property. The amphiphilic peptides were proved to distribute evenly and stably in the nanofibers due to the hydrophobic interaction with PLA and to improve the cell adhesion property. In addition, nanofiber membrane conduits for nerve tissue regeneration was developed and evaluated in vitro and in vivo.
