抄録
Direct and real–time visualization of biological molecules at work is a straightforward and powerful approach to understanding the mechanisms of biomolecular processes. Recent advances of high–speed atomic force microscopy (HS–AFM) have opened new possibility to visualize dynamic events of label–free proteins under physiological conditions, at subsecond to sub–100 ms temporal and submolecular resolution. Here we overview the technical challenges to fast and low–invasive imaging of biomolecules with AFM. Then we illustrate some recent results demonstrating capability of the high–speed AFM for observing dynamic events of membrane related proteins such as conformational changes and two–dimensional crystal dynamics.
