抄録
We have developed a substrate surface that enables the photo–patterning of cells with single–cell precision and have been conducting research to acquire image data on molecular and cellular behaviors that were previously unattainable due to technical challenges. In these studies, we employed polyethylene glycol–lipids (PEG–lipids), a polymer material that interacts with cell membranes. We succeeded in converting PEG–lipids into photoresponsive materials and developed three types of photoresponsive cell–adhesive surfaces: photocleavable, photoactivatable, and photoswitchable. In this paper, we introduce the development of these surfaces and their applications in membrane protein localization analysis on non–adherent single cells and microscopic analysis of intracellular faces of intact cell membranes.
