Studies of Electrophoresis of Charged Organic Substances through Charged and Non-charged Porous Membranes

Abstract

Two types of charged porous membranes called CMN and CMA were obtained by co-polymerization of styrene and divinylbenzene. CMN membranes were prepared by using nitrilrubber which has high performance of acid resistance, and also CMA by using acrylicrubber which has no acid resistance. CMA membranes were more porous than CMN membranes. Amino acid could permeate through CMA and CMN membranes. CMN membrane had good performance of electrophoretic separation of amino acids from mixture solution, comparing with CMA membrane. Egg albumin could not pass through CMN and commercially available ion exchange membranes but permeated through CMA membrane. Separation seems to depend on a poresize of the membrane. The suitable pore size of permeation by electrophoresis of egg albumin was estimated to be 0.05 gm using many types of commercially available non-charged micro porous membranes. The pore size of CMA membrane seems to be bigger than 0.05 gm.