抄録
This mini-review focuses on the application of liposomes in two fundamental techniques of biotechnology, gene transfer and extraction of membrane proteins. Three major methods of gene transfer using liposomal carrier, namely, fusogenic HVJ-liposomes, fusogenic PEO-modified liposomes and pH-sensitive liposomes, are described together with cationic “liposomes” for transfection. For the membrane protein extraction, the background and the recent development of the technique are explained. In addition, recent reports of stimulation of T-cells with liposome-incorporated gangliosides and chaperone-like behavior of liposomes are briefly noted.
