High Throughput Sample Preparation using Ultrafiltration in Multiwell Plate Format : Post-Sequencing Cleanup of Dye Terminator Sequencing Reactions for Capillary Electrophoresis

抄録

A unique new method is described showing the use of microfiltration and ultrafiltration membranes in conjunction with gel permeation in 96 well plates. Using this technique it is possible to clean up DNA sequencing reactions to a level of template DNA purity that makes high DNA loading onto capillary electrophoresis (CE) sequencers possible. Loadings as high as 10, 000 ng allow sequencing on CE to be equal in performance to gel slab electrophoresis. This technique replaces alcohol precipitation for sequencing reaction cleanup with an easily automated process taking 1/6 the time.