細胞膜リン脂質のダイナミズムを介した膜と細胞骨格のクロストーク

抄録

Phosphatidylethanolamine (PE) is a major membrane phospholipid that is mainly localized in the internal leaflet of the plasma membrane. We have shown that PE was exposed on the external leaflet of the cleavage furrow as a result of enhanced transbilayer movement of the phospholipids during cytokinesis. To further investigate the role of PE in cytokinesis, we employed two different approaches : manipulation of cell surface PE by a PE-binding peptide, and the establishment of a mutant cell line specifically defective in PE biosynthesis. Both approaches clearly demonstrate that surface-exposed PE is essential for disassembly of the contractile ring at the final stage of cytokinesis. Based on these findings, we proposed that the transbilayer redistribution of PE plays a critical role in mediating coordinated movements between the cytoskeleton and the plasma membrane that are required for the proper progression of cytokinesis.