細胞シート工学 : 肺細胞の培養と再生医工学への応用

抄録

Toward the next generation tissue engineering, we have developed a novel technology called “cells sheet engineering”. By covalently grafting a temperature-responsive polymer, temperature-responsive culture surfaces are prepared in order to harvest cell sheets non-invasively without proteolytic enzymes such as trypsin. Since harvested cell sheets retain extracellular matrix on the basal surface of cell sheets, cell sheets readily adhere to another surfaces such as wound beds and apical cell surfaces. The technology has been clinically applied in part and would open the way to reconstruct complicated tissue structures including lung alveoli.