酵素固定化膜の性能評価法
1981 年 6 巻 3 号 p. 10-20
詳細
1981 年 6 巻 3 号 p. 10-20
Considerable worldwide interest has arisen in the use of immobilized biocatalysts in industrial processes and analytical chemistry. Many methods for immobilizing enzyme have been developed. Simple immobilization techniques and inexpensive carriers are desirable for practical uses of immobilized biocatalysts.
This paper summarizes the preparation of immobilized enzymes, especially, enzyme-membranes and the characteristics of immobilized enzyme prepared. Application of enzyme-membranes to bioelectrochemical sensors such as enzyme, organelle, immuno, and microbial sensors and the principle of the bioelectrochemical sensors are described. Furthermore, generation of a cofactor such as NAD in an enzyme-membrane, properties and application of asparaginase-collagen membrane, and bacteriolysis by immobilized enzymes are also described.
An electrochemical method for evaluating enzyme-membrane characteristics is proposed by the authors. The method bases on monitoring the properties of enzyme-membrane attached directly on the devices, such as oxygen, pH and CO 2 electrodes. The factors affected the properties (for example stability) of enzyme membranes are also discussed here.
