We investigated the change in renal brush border membrane (BBM) in the experimental Fanconi's syndrome rabbit induced by maleic acid administration.
Male albino rabbits, weighing 2.0-2.5kg, were given an intravenous injection of 50-500mg/kg B.W. of neutralized maleic acid. One hour later, BBM was isolated by the continuous sucrose density gradient centrifugation method of Sacktor et al.. The activities and distribution pattern of marker enzymes (trehalase, γ-glutamyl transpeptidase and alkaline phosphatase) were not affected by maleic acid administration.
By the electron microscopic observation showed that BBM isolated from rabbits administrated with maleic acid was a closed vesicle of membrane as well as BBM from the control rabbit.
The SDS-polyacrylamide gel electrophoresis pattern of BBM proteins isolated from rabbit administrated with maleic acid was same to that from the control rabbit.
These data suggest that maleic acid induce Fanconi's syndrome by affecting the other site than BBM.
