Abstract
This article deals with the analysis of D-glucose and several drug transport systems using plasma membrane vesicles derived from fibroblasts and renal epithelial cells. Membrane vesicles isolated from non-transformed fibroblasts and from transformed cells displayed carrier-mediated and stereospecific transport of D-glucose, and retained many of the features of the increased glucose transport observed in whole cells in association with viral transformation. The transport systems in brush border and basolateral membranes isolated from kidney cortex differ from one another : brush border membranes catalyze secondary active transport of D-glucose by an electrogenic Na+ symport mechanism and carrier-mediated transport of amino-β-lactam antibiotics, and basolateral membranes catalyze carrier-mediated transport for p-aminohippurate (organic anion).
