Abstract
Flash photolysis technique is one of the dynamical methods to determine kinetic parameters or to assign the reaction intermediate with short half life. Compared with other tachniques such as stopped flow and temperature jump methods, it has advantages in probing non-invasively the photosensitive pigments in tissues, cells, organella, and so on. This technique has been applied to studies on photosynthesis, visual pigments, hemoproteins, and etc. In this article we reviewed the application of this method to the analyses of membrane-bound hemoproteins such as cytochrome oxidase and cytochrome P450.
