Acute toxicity of a mixture of warfarin and vitamin D_3 to laboratory rats

Abstract

The toxic response of albino rats (SD strain, Rattus norvegicus) to warfarin, vitamin D_3 and mixtures of the two compounds was investigated. Acute oral LD_<50> values of the test rats determined by single oral administration of some mixtures of warfarin and vitamin D_3 were shown as follows : warfarin+vitamin D_3 (1 : 4) : 4.3,17.0mg/kg; warfarin+vitamin D_3 (1 : 2) : 9.7,19.5mg/kg; and warfarin+vitamin D_3 (1 : 1) : 12.0,12.0mg/kg. On the other hand, acute oral LD_<50> values of warfarin and vitamin D_3 applied separately, were 61.1 and 44.7mg/kg, respectively. There was no significant difference between male and female in the acute toxicities of warfarin, vitamin D_3 and mixtures of the two. Synergistic effects between warfarin and vitamin D_3 were apparently observed in oral acute toxicities to rats.