Abstract
First-instar larvae of Aedes albopictus naturally infected with a rickettsial endosymbiont (Wolbachia pipientis) were treated with tetracycline. The treatment successfully removed Wolbachia and resulted in establishment of a Wolbachia-free strain. Testing for the expression of cytoplasmic incompatibility in the cured and infected strains showed complete incompatibility only in crosses between the infected males and the cured females, which are typical crosses that express cytoplasmic incompatibility in other species.
