2003 Volume 10 Issue 1 Pages 21-31
The PCR-Luminex method using fluorescence microsphere was developed for high-resolution HLAA, HLA-B and HLA-DRB1 genotyping in the Japanese population. This genotyping method allowing to define all the possible combinations of alleles at each loci existing in Japanese at the four-digital level except for A*2402, 2601and A*2404, 2605 was accomplished using 33 probes for HLA-A, 46 probes for HLA-B and 44 probes for HLA-DRB1. This method was evaluated with 138 Japanese samples and concordance rates of this typing to the predetermined assignments were 99.3% at the HLA-A and HLA-B loci, and 100% at the HLA-DRB1 locus. Collectively, the PCR-Luminex method is a simple, accurate and high-through put (HLA-A, -B and DRB1 typing of 47 samples for 5 hours) genotyping system offering the efficient routine platform in the HLA field.
