Detection of HLA−A2 and−B46 natural antibodies in healthy Japanese male blood donors.

Abstract

We have examined 10,000 blood donors to perform the extensive study for screening the possible HLAantibodies by LCT (lymphocyte cytotoxicity test) assay. Two male donors were found to possess monospecific natural antibodies against HLA-B46 and -A2. Immunoglobulin subclass of both natural antibodies was IgM. Both donors have not experienced any blood transfusion nor transplantation. In addition, any family members of the donor positive for HLA-B46 antibody did not possess HLA-B46 antigen nor antibody. It is likely that HLA natural antibodies are more prevalent than formerly supposed. HLA antibody screening including male donors or nonpregnant female donors will provide further insights on the characteirization of HLA natural antibodies.