A Nuclepore Filter Bag for Preparation of Small Biological Specimens for Scanning Electron Microscopy

Abstract

A method for the preparation of minute, delicate and dilute suspended organisms for scanning electron microscopy (SEM) by using of Nuclepore filter bags (NFB) is described. NFBs are easily made by welding with a heat sealer and are inert with solvents used in the preparation of specimens for SEM. The use of NFB avoids the risk of unintentional air-drying, physical damage, contamination, and loss of specimens during preparation. Specimens prepared by NFB show fine details of three-dimensional surface structure.