Abstract
Quantitative DNA-DNA hybridization and DNA base composition (mole % G+C) were applied to bacterial flora analysis in a eutrophic lake. Biochemical and morphological characterization by API 20 NE revealed 26 isolates from the eutrophic lake consisting of one major and three minor phenotypic groups. The isolates of each phenotypic group had a similar mole % G+C. DNA-DNA hybridization of these isolates showed that these phenotypic groups belonged to three genetic clusters. Two clusters were represented by each phenotypic group, but one of them included two strains phenotypically unidentified by API tests. Another cluster was consisted of two phenotypic groups and two unidentified strains. Thus, DNA analysis revealed different profile of the bacterial flora from the conventional method, adding genetic information of the constituents of bacterial community.
