2021 年 5 巻 1 号 p. 38-46
The etiology and pathogenesis of interstitial pneumonia have not been fully elucidated. A proteomics-based approach using two-dimensional electrophoresis/in-gel digestion/matrix-assisted laser desorption time-of flight mass spectrometry (MALDI-TOF MS) was applied to identify new candidate biomarkers in whole lung extract from the bleomycin-induced interstitial pneumonia model mouse. First, the mouse lung extract was subjected to two-dimensional electrophoresis and then the bleomycin-induced interstitial pneumonia (BLM-IP) specific spots underwent in-gel digestion. The proteins in the specific spots were identified by MALDI-TOF MS and MSMS analyses. Nine proteins including anti-oxidative stress proteins, transgelin-2 (TGLN-2), vimentin, transthyretin, fatty acid-binding protein, and sepiapterin reductase were identified by MALDI-TOF MS and MSMS. The expression levels of these 9 proteins were clearly up-regulated when compared with those of the healthy mouse lung.
In conclusion, the proteomics-based approach enabled us to identify nine up-regulated proteins, especially TGLN-2, in the BLM-IP model mouse. The up-regulated modified TGLN-2 might be an early marker of smooth muscle cells differentiation induced thicken of the alveolar walls and play an important role in pulmonary fibrogenesis.
