Comprehensive analysis of fatty acid metabolites produced by gut microbiota using LC-MS/MS-based lipidomics

抄録

The saturation metabolism of polyunsaturated fatty acids represents a mode of detoxification metabolism by the gut microbiota. It generates a series of functional fatty acids, such as hydroxy fatty acids (e.g., 10-hydroxy-cis-12-octadecenoic acid (HYA) from linoleic acid), oxo fatty acids, conjugated fatty acids, and trans fatty acids. In this study, we prepared 45 synthetic standards, including HYA and related fatty acid metabolites, and developed liquid chromatography-tandem mass spectrometry-based targeted lipidomics. This method was successfully applied for the comprehensive analysis of the gut microbiota-dependent production of fatty acid metabolites in mouse feces, plasma, and tissues under different dietary conditions.