Quantification of threonine-containing lysophospholipids using ESI-LC-MS/MS in mouse tissues

抄録

Lysophosphatidylthreonine (LysoPT) is a potent inducer of mast cell degranulation that was previously identified as an analog of a bioactive lysophospholipid, lysophoshatidylserine (LysoPS). However, it remains to be solved if LysoPT is present in vivo. In the present study, we tried to detect LysoPT in biological samples using liquid chromatography/tandem mass spectrometry (LC-MS/MS) and examined its tissue distribution. In several mouse tissues, we detected LysoPT as a mixture of LysoPT with several different fatty acid chains, i.e., LysoPT species. C18:0-LysoPT was a major LysoPT species in various tissues and plasma with the highest expression in the stomach. The fatty acid species and tissue distributions of LysoPS and LysoPT were similar, suggesting that the two lysophospholipids share a common synthetic pathway. The present study indicates that LysoPT behaves as an endogenous lysophospholipid mediator.