Abstract
There is a pond in Miyazaki Jingu Shrine in the center of Miyazaki City, Miyazaki Prefecture. This pond used to have water flowing from a nearby river, but since the water was cut off several decades ago, it is now drawn from a well. The biota of this pond has never been investigated before.
In October of the 5th year of Reiwa, construction work was carried out to clean the bottom of the pond, and I took this opportunity to investigate the biota. I also investigated what kind of invasive species are inhabited. As a result, several types of invasive species were found. In addition, I requested DNA analysis of the terrapin (Pelodiscus japonicus) because it was indistinguishable from the invasive species(Pelodiscus sinensis). As a result, the nuclear DNA was from Pelodiscus japonicus, but the haplotype of mitochondrial DNA was clearly from Pelodiscus sinensis.