Detection of Aspergillus novoparasiticus from Japanese sugarcane field by the dichlorvos-ammonia (DV-AM) method with single colony AM assay

Abstract

  The dichlorvos-ammonia (DV-AM) method followed by single colony AM assay was applied for re-screening of aflatoxigenic fungi from soil samples from sugarcane field in Okinawa, Japan. In previous study, we obtained two aflatoxigenic isolates of Aspergillus pseudonomius located in Aspergillus nomius clade from one of soil samples. In this study, we re-screened the same soil sample and found an isolate with different morphology and toxigenic profile. Molecular phylogenic analyses of the isolate indicated that it is a closely related species of A. novoparasiticus located in Aspergillus flavus clade. This is the first report of this species of Aspergilli in Japan. Thus, the DV-AM method with single colony AM assay can be used as a tool to elucidate the diversity of aflatoxigenic fungi colonizing in the field soil.