2023 Volume 73 Issue 2 Pages 61-63
In a previous study, an in vitro cell-free assay of translation inhibition by H-2 toxin, HT-2 toxin, and deoxynivalenol using a commercially available kit was developed. They showed significant inhibition of the translation in the system. For further evaluation, I examined three additional modified deoxynivalenol compounds, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, and deoxynivalenol-3-glucoside, by the in vitro cell-free assay in this study. 15-acetyldeoxynivalenol inhibited translation as well as deoxynivalenol in the assay. On the other hand, 3-acetyldeoxynivalenol and deoxynivalenol-3-glucoside show around 100 times lower and no inhibitory activities, respectively, on the translation in the assay. These data are consistent with previous studies, suggesting that the assay is useful. It is hoped that it will be applied to other trichothecenes in the future.
