Comparison of secondary metabolite production in the sterigmatocystin and austinol cluster deletion strains in Aspergillus nidulans

Koko Hanazawa; Yosuke Nakamura; Ngoc-Hung Nguyen; Natsuki Hagihara; Phuong-Thao Nguyen; Thi Huynh Tram Le; Kiminori Shimizu

doi:10.2520/myco.73-2-1

This article has now been updated. Please use the final version.

Comparison of secondary metabolite production in the sterigmatocystin and austinol cluster deletion strains in Aspergillus nidulans

Koko Hanazawa, Yosuke Nakamura, Ngoc-Hung Nguyen, Natsuki Hagihara, Phuong-Thao Nguyen, Thi Huynh Tram Le, Kiminori Shimizu

Author information

Koko Hanazawa
Department of Biological Science and Technology, Tokyo University of Science
Yosuke Nakamura
Department of Biological Science and Technology, Tokyo University of Science
Ngoc-Hung Nguyen
Department of Biological Science and Technology, Tokyo University of Science
Present address: Department of Computational Biology and Medical Science, Graduate School of Frontier Sciences, The University of Tokyo
Natsuki Hagihara
Department of Biological Science and Technology, Tokyo University of Science
Phuong-Thao Nguyen
Department of Biological Science and Technology, Tokyo University of Science
Thi Huynh Tram Le
Department of Biological Science and Technology, Tokyo University of Science
Present address: Department of Environmental Engineering, Faculty of Environment and Natural Resources, Ho Chi Minh City University of Technology
Kiminori Shimizu
Department of Biological Science and Technology, Tokyo University of Science
Medical Mycology Research Center, Chiba University

Keywords: biosynthetic gene cluster, dehydroaustinol, gene deletion, secondary metabolism

JOURNAL FREE ACCESS Advance online publication

Article ID: 73-2-1

DOI https://doi.org/10.2520/myco.73-2-1

The final version of this article is now available: Vol. 73 (2023), No. 2 pp. 55-60

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Abstract

A filamentous fungus Aspergillus nidulans produces a mycotoxin sterigmatocystin (ST), in addition to other secondary metabolites (SMs). SM biosynthetic pathways share common precursor substances. However, how the loss of one SM affects the production of other SMs remains unclear. In this study, we deleted 8 biosynthetic gene clusters (BGCs) or a polyketide synthase gene wA, respectively, and analyzed ST production in those deletion strains. In aus1Δ lacking austinol (AUS) cluster A, ST production was increased compared to the control strain. However, in aus1Δ aus2Δ lacking both AUS cluster A and B, ST production was comparable to the control. In STΔ lacking ST cluster, AUS and dehydroaustinol (DAUS) production was comparable to the control.

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