Development of enzyme-linked immunosorbent assay (ELISA) for T-2 toxin using monoclonal antibodies

Abstract

Two monoclonal antibodies (7 D 4, 6 E 9) reactive with T-2 toxin (T-2) were prepared. The antibody 7 D 4 was also reactive with T-2 hemisuccinate (T-2 HS) and acetyl T-2 toxin, but less reactive with HT-2, 3'-OH-T-2, and 3'-OH-HT-2 toxins. By using the antibody 7 D 4 in the indirect competitive ELISA method, the least detectable limit for T-2 was about 25 pg per assay.